The proportion of prostate area positive for Shh staining also increased in TRAMP controls with an increase in the aggressiveness of the tumor; specifically, there was an increase of ~1.3 fold (LGPIN), ~2.2 fold (HGPIN), ~3.6 fold (WD), and ~4.8 fold (for both MD and PD, p ≤ 0.001) (Figure 7B, upper right panel) compared to WT controls. The gene discussed is SHH; the disease is neoplasm.