Consistent with previous microarray and RNA seq gene expression data on primary RB tumor tissues [56,57,58], candidate coding (RB1, E2F3, MYCN, MDM2, KIF14, MDM4, DEK, CDH11, CEP170, SIX1, SIX4, and SYK) and ncRNAs (miR-17-5p, 20a-5p, 324-5p, 182-5p, 181a-3p, 191-3p, 451b, hsa-let 7a, let 7e,) involved in RB tumorigenesis were also found to be dysregulated in RB sEVs. The gene discussed is SIX4; the disease is neoplasm.