To investigate more in depth the role of CK1α silencing in the third co-culture model and to explain the unexpected elevation in RUNX2 levels in PCs after co-silencing CK1α in MM and MSC-hTERT cells (Figure 3, model 3), we modified this third model by physically separating MM and MSC cells using a transwell system. Here, RUNX2 is linked to Miyoshi myopathy.