In our animal experiments, we did not demonstrate whether demethylation therapy with PRKCZ was effective in suppressing tumor growth because this function had been reported by Biktasova A et al. These researchers proposed that the application of 5-aza could inhibit HPV+ HNSCC tumor growth and mouse blood vessel invasion by repressing the expression and activity of matrix metalloproteinases [67]. Here, PRKCZ is linked to head and neck squamous cell carcinoma.