As illustrated in Table 1, compared to the normal control group (NC), there were significant increases (p < 0.001) in levels of serum AST, ALT, total bilirubin, direct bilirubin, and AFP with a significant decrease in the level of serum albumin upon feeding the experimental rats with HSHF diet in NASH group. The gene discussed is GPT; the disease is metabolic dysfunction-associated steatohepatitis.