TLR4 and metabolic dysfunction-associated steatotic liver disease: Therefore, the aim of the present study was to evaluate the effects of calcium-sulphate-bicarbonate water (Wcsb) in a mouse model of NAFLD (i.e., methionine-choline-deficient diet–MCD) and, in particular, to assess: (i) eventual improvements to liver histopathology (i.e., liver steatosis, inflammation and fibrosis); (ii) modification of LPS liver localization; (iii) TLR4 pathway activation; and iv) improvements of terminal ileum histopathology and modification of occludin positivity in enterocytes.