HFD significantly increased body weight, liver weight, AST and ALT levels, macrophage infiltration levels in liver, pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α), the NAFLD activity score, liver steatosis, lobular inflammation, hepatocyte ballooning, liver collagen area, liver TGF-β1, and type I collagen expression levels. This evidence concerns the gene TNF and fatty liver disease.