VDAC1 and amyotrophic lateral sclerosis: In order to determine whether the oligomeric state of VDAC1 is affected in ALS pathogenesis, we isolated spinal cord mitochondria from non-transgenic, transgenic SOD1WT, and mutant SOD1G93A rats (Figure 3B,C) and spinal cord mitochondria from non-transgenic and mutant SOD1G93A mice (Figure 3D–G) and performed chemical crosslinking with two different crosslinking reagents.