A transgenic model of zebrafish glioblastoma was generated by Ju et al. in 2009, by employing a binary transgenic approach Gal4VP16-UAS system, which co-expressed an oncogenic zebrafish Smoa1 with a constitutively active human AKT1, which induced serval types of tumours with a higher incidence of glioblastoma and astrocytoma [120]. Here, AKT1 is linked to glioblastoma.