Therefore, we checked the repression effect of atropine on EMT inducers as compared with the effect of vit D. The MDA-MB-231 and T47D breast cancer cell lines were cultured in the presence of atropine (25 μmol/L) or DMSO with or without TGF-β (10 ng/mL) induction and Vit-D (10 nM)-supplemented cells (Figure 5). This evidence concerns the gene TGFB1 and breast carcinoma.