In addition, we sensibilized (at nontoxic concentrations) the neurons with a lysosomal glucocerebrosidase (GCase) inhibitor toxin ([conduritol-B-epoxide CBE]) in order to increase the α-syn lysosomal clearance deficit (also found in some genetic cases of Parkinson’s disease (Glucocerebrosidase (GBA1) gene mutation)). Here, GBA1 is linked to Parkinson disease.