The inflammatory cytokine is an important index for evaluating the severity of colitis, so we screened the expressions of pro-inflammatory cytokines (IL-1β, IL-6, IL-17, and TNFα) and anti-inflammatory cytokines (IL-10 and TGFβ) using qPCR analysis in colon tissues of mice between control and AOM/DSS groups. This evidence concerns the gene TNF and colitis.