In summary, immunolabeling detected the presence of neuronal (TuJ1 80–90%, MAP2 80–90%) and motor neuron specific markers (SMI32 70–75%) in the cells without significant differences in neuron morphologies between wildtype controls (n = 3 subjects, 1 clone each) and ALS mutants (SOD1 n = 2 subjects, 1 clone each; TDP43 n = 2 subjects, 2 clones of one subject and 1 clone of second subject). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.