First, Western blot analysis has again confirmed that miR-99b-5p negatively regulates mTOR protein expression in these cancer cells, evident from a generalized reduction in total mTOR (combined protein levels of cytoplasmic and nuclear mTOR) and a significant decrease in nuclear mTOR (and nuclear pmTOR) expression in miR-99b-5p vs. NS transfected cells (Figure 6A, and Supplementary Figure S3). The gene discussed is MTOR; the disease is cancer.