MTOR and posterior cortical atrophy: To assess our hypothesis that the AA PCa aggressiveness may be due to the overall upregulation of nuclear mTOR and pmTOR, nonsense/scrambled RNA (NS) or miR-99b-5p mimic was transfected to the EA PCa and AA PCa cell lines then followed by examining the subcellular distributions/expression levels of mTOR and pmTOR by using immunofluorescence assays and Western blot analysis.