First, the Western blot analysis has shown that mTOR is upregulated in colon cancer (HT-29 and SW620) vs. normal colon (FHC) cells, in breast cancer (MDA MB 231, MCF-7) vs. normal breast (HMEC) cells, in lung cancer (A549 and H1299) (Figure 4C). This evidence concerns the gene MTOR and breast cancer.