The TLR3 rs3775296 T allele and the TLR4 rs4986790 G allele were more frequent in patients with EMI than in those without extramedullary metastases, whereas the TLR3 3775291 A allele and the TLR9 rs187084 C allele tended to be less frequent in patients with EMI than in those without extramedullary manifestations of AML. This evidence concerns the gene TLR9 and acute myeloid leukemia.