The progressive gene expression connectivity among LOX, LOXL1, and LOXL3 and matrisome-related genes from LGG-IDHmut, LGG-IDHwt, and through to GBM, reinforce the impact of ECM components contributing for matrix stiffness on the malignant progression and prognosis of astrocytoma. This evidence concerns the gene LOXL3 and astrocytoma (excluding glioblastoma).