The progressive gene expression connectivity among LOX, LOXL1, and LOXL3 and matrisome-related genes from LGG-IDHmut, LGG-IDHwt, and through to GBM, reinforce the impact of ECM components contributing for matrix stiffness on the malignant progression and prognosis of astrocytoma. The gene discussed is LOX; the disease is astrocytoma (excluding glioblastoma).