As shown in Fig. 6, median MSH4 FC was significantly lower with LN metastasis (0.069, IQR:0.28) than without LN metastasis (0.27, IQR:0.53) by 74.4% (p = 0.019), late stage (0.143, IQR: 0.259) than early stage (0.539, IQR:0.67) by 73.47% (p = 0.002), tumor size ≥ 4 cm (0.138, IQR:0.34) than tumor size < 4 cm (0.325, IQR:0.69) by 57.54% (p = 0.012) and MIBC (0.139, IQR:0.23) than NMIBC (0.65, IQR:0.59) by 78.61% (p < 0.001). The gene discussed is MSH4; the disease is neoplasm.