At endpoint, Trp53(+/+) tumours had the highest immune cell infiltration (comprising all the four immune cell populations CD4+, CD8+, B cells and Tregs), followed by Trp53(−/−), Trp53(R172H/R172H) and Trp53(R245W/R245W) cohorts, respectively (Fig. 4c, d). Here, CD4 is linked to neoplasm.