In agreement with previous studies showing that MAD2L2 is essential for interstrand crosslink repair37,38, MAD2L2 depletion sensitized HeLa cells to the DNA cross-linking agents cisplatin (CIS) and mitomycin C (MMC) (Fig. 1a, b, Supplementary Fig. 1d). This evidence concerns the gene MAD2L2 and in situ carcinoma.