Given the possibility that hemodynamic measurements could be confounded by insulin‐induced peripheral vasodilation, mediated by endothelial nitric oxide synthase (eNOS) activation and nitric oxide (NO) production, we co‐infused animals with l‐NAME (50 mg/kg bolus i.p. followed by 1 mg/kg/min) for the duration of the clamps to determine whether the effect of hyperinsulinemia on LV developed pressure could be prevented. This evidence concerns the gene NOS3 and hyperinsulinism.