Research has shown that HT29 colon cancer cells with high Wnt3a expression have a stronger ability to form tubular structures in three-dimensional culture, and the expression of endothelial phenotype-related proteins, such as vascular endothelial growth factor 2 (VEGFR2) and VE-cadherin, are increased [45]. This evidence concerns the gene CDH5 and malignant colon neoplasm.