It is worth mentioning that the mouse NASH model induced by Western diet and CCl4 is more advantageous in mimicking the histological, immunological, and transcriptomic features of human NASH than the HFHC model used in the previous study (37, 38), By using the mouse NASH model, we found the effect of TMAO on protecting the integrity of hepatic sinusoidal endothelium and identified the ATP1B1 instead of the canonical targets and pathways, revealing a new insight into microbiota-metabolite-vascular microenvironment crosstalk. The gene discussed is ATP1B1; the disease is metabolic dysfunction-associated steatohepatitis.