Consistent with the concept of AIH, we found that the dose of LPS can have qualitative effects on the diversity of the response; quadruple positive and TNF negative triple positive (TNF-proIL1β+IL6+NOS2+) cells appear prominently at higher doses of LPS (100, 1000ng/ml;Figure 1B), while quadruple negative (TNF-pro-IL1β-IL6-NOS2-) sub-populations and single positive cells for TNF (TNF+pro-IL1β-IL6-NOS2-) appear at lower doses (1, 10ng/ml;Figure 1B). This evidence concerns the gene NOS2 and autoimmune hepatitis.