A previous report by Ardianaet al. showed that exposure to cigarette smoke decrease e-NOS level in the aorta.25 A decline in e-NOS concentration led to not only increase in expression of adhesion molecules and vascular tone but also initiate inflammation coagulation cascade.26 As a result, aortic intima-medial is thickening in the early step of atherosclerosis. This evidence concerns the gene NOS3 and atherosclerosis.