Sethiet al. claimed that thymoquinone at the dose of 10 μM on 4 hours incubation can suppress NF-κB activation in human leukemia myeloid cells and human embryonic kidney cells exposed to TNF- α.33 Suppression of NF-κB activation reduce the production of pro-inflammatory cytokines such as TNF-α, IL-1β, IL-6, IL-8, TLR2 and TLR4 which then stimulate M1 to M2 macrophage polarization.29 This evidence concerns the gene TNF and leukemia.