In the qRT-PCR experiment, mRNA expression levels of BAK1, CHMP4B, and DHX9 were significantly higher in all experimental HCC cell lines than in normal one while GSDME and CHMP4A were only higher in three and one HCC cell lines, respectively, (Welch's t-test, P < 0.05, Figures 7(d)–7(h)). This evidence concerns the gene GSDME and hepatocellular carcinoma.