To this task, large EVs and small EVs (derived from the 2 K and 10 K fractions, respectively) constitutively shed by U87 transfected with AQP4-tetramers or AQP4-OAPs or with the empty vector used as a control (Mock) were added to pre-seeded glioma (U87) untransfected recipient cells and analysed by immunofluorescence after 48 h. The gene discussed is AQP4; the disease is glioma.