To further clarify the role of RCAN1 in AKI, we crossed RCAN1 floxed allele mice with TECs-specific Cdh16-Cre mice to generate TECs-specific RCAN1 knockout mice, and found that RCAN1 knockout reduced renal injury, mitochondrial dysfunction, and apoptosis in AKI. This evidence concerns the gene CDH16 and acute kidney injury.