The final selected eight genes were NFE2L2, CSMD1, CREBBP, KALRN, PRUNE2, NRXN1, AKAP9, and FREM2 (Fig. 7a), among which five genes (NFE2L242, CSMD143, CREBBP44, PRUNE245, and AKAP946) had been reported as tumor-suppressing gene or with dominant gain-of-function mutational hotspots in tumors. Here, PRUNE2 is linked to neoplasm.