GPC3 and neoplasm: We selected PC10 and NCI-H446 cells to represent high GPC3-expressing tumours, with 7.22 × 104 or 7.16 × 104 antigen-binding capacity (ABC) per cell, respectively, and MKN74 and MKN45 cells to represent low GPC3-expressing tumours, with 2.49 × 103 or 4.86 × 103 ABC per cell, respectively (Fig. 1a).