Moreover, virus titers in SPHK2 overexpressed A549 cells were increased by approximately 1.2-log (P<0.0001) at 24 h post infection, while the viral titers in SPHK2 knockout A549 cells dropped more than 1.3-log (P<0.01), compared to that in the WT A549 cells (Fig 3B). This evidence concerns the gene SPHK2 and infection.