Further investigation of microglia‐related superpixel correlations with multiple linear regression revealed that y‐intercepts (disease: y = 10.5437, control: y = 1.9610), but not slopes (disease: a = 0.3733, control: a = 0.4162), of the linear models of Iba1+ versus CD68+ superpixels were significantly different between C9‐ALS and control cases (p < 0.001), indicating elevated microglial activation in C9‐ALS (Figure 5A). This evidence concerns the gene AIF1 and amyotrophic lateral sclerosis.