In this study, the expression level, diagnostic efficiency, and prognostic significance of HSF1 in AML, as well as the correlations between HSF1 and clinical parameters, were investigated and validated based on The Cancer Genome Atlas (TCGA), Genotype–Tissue Expression (GTEx), Gene Expression Omnibus (GEO), Cancer Cell Line Encyclopedia (CCLE), and Beat AML data viewer (Vizome). This evidence concerns the gene HSF1 and cancer.