We were able to introduce mutations into the previously published and commonly targeted HEK site 3 (HEK3) locus (CTT-sequence insertion) (Anzalone et al., 2019), as well as two additional PD-associated mutations into the α-Synuclein (SNCA) locus (A30P [G88C]; Krüger et al., 1998; and A53T [G209A] Polymeropoulos et al., 1997; Figure 1—figure supplement 1B, C) with editing efficiencies comparable to the LRRK2 locus (Figure 1D and E; quantified as pure prime editing efficiencies [PPE] as defined in Petri et al., 2021). Here, LRRK2 is linked to Parkinson disease.