To quantify levels of DVG and full-length SINV genomic RNA during infection, we designed a BRYT Green-based qPCR assay using primer sets that either span the deleted portion of the SINV genome for DVG amplification or are within the deleted portion (nsP3 gene) for SINV genomic RNA detection (Fig. 4C). The gene discussed is SH2D3C; the disease is infection.