Immunohistochemical studies were performed using DAPI and calbindin labeling at the end of the protocol (P12; N group = 3 males; IH group = 3 males and 1 female) or 9 days later (P21; N group = 1 male and 2 females; IH group = 2 males-1 female) to analyze the short and long-term effects of IH on cerebellar histology. Here, CALB1 is linked to isolated hemihyperplasia.