Consistently, immunoblotting and IHC analysis in clinical HCC tissue exhibited decreased expression for TRIM36 and increased expression for active β-catenin, β-catenin and Ki-67 compared to adjacent control tissue, further verifying the connection between TRIM36 and the Wnt/β-catenin pathway (Fig. 6a–c). This evidence concerns the gene TRIM36 and hepatocellular carcinoma.