The reintroduction of WT ASXL1 protein restored H3K27me3 levels and suppressed HOXA gene expression in leukemia cells with mutated ASXL1. However, neither an interaction between ASXL1 and the PRC1 component BMI1 nor changes in H2AK119Ub1 levels with ASXL1 loss were evident in the AML cell lines evaluated (SET2 and/or UKE1 cells) [94]. This evidence concerns the gene ASXL1 and acute myeloid leukemia.