Second, Piezo1 may promote integrin activation via FAK signaling: a Piezo1-dependent feedforward between glioma mechanotransduction and tissue stiffening can regulate focal adhesion assembly and activate integrin-focal adhesion signaling in gliomas44; blood flow perturbations can promote Piezo1-mediated integrin activation and FAK-dependent NF-κB activation in endothelial cells; and Piezo1-depleted endothelial cells show pathologically reduced integrin activation33,57. This evidence concerns the gene PTK2 and glioma.