On the other hand, miR-25-3p shuttled by exosomes in colorectal cancer can be transferred to vascular endothelial cells to mediate the expression of ZO-1 and occludin by targeting Kruppel-like factors (KLFs) (41), and miR-16 also serves as a regulator of KLF4 (42), so it may be speculated that miR-16 affects ZO-1 and occludin in a KLF4-dependent manner. This evidence concerns the gene KLF4 and colorectal cancer.