AKT1 and infection: To elucidate whether AKT, the upstream molecule of mTORC1, was involved in the activation of mTORC1 signaling in WSSV infection, an injection of MK2206, a specific AKT inhibitor, was administered, and activation of mTORC1 (using phosphorylation of 4EBP1 as the indicator) and WSSV replication (using Vp28 expression as an indicator) were analyzed.