During viral infection, TRIM25-R54P enriches for all TRIM25-WT interactors in addition to 16 unique interactors (Tables 3 and 4), suggesting an effective “substrate trap.” Interestingly, we found that the number of TRIM25 interactors drastically decreases during viral infection for both TRIM25-WT (29 to 7 interactors; Tables 2 and 4) and TRIM25-R54P (38 to 23 interactors; Tables 1 and 3). Here, TRIM25 is linked to viral infectious disease.