TNNI3 and acute myocardial infarction: Thepresence of the three proteins in the sample turned the TL brown.The assay was shown to be quantifiable using RGB analysis with a smartphonecamera.135 Similarly, Zhang et al. havedeveloped a multiplexing strategy for the detection of three cardiacdisease biomarkers for the early detection of acute myocardial infarctions[creatine kinase-MB isoenzymes (CK-MB), cardiac troponin I (cTnI),and myoglobin] in a single TL, using SERS signal transduction (Figure 11).