By prioritizing good-quality, non-silent, and very rare (MAF ≤ 0.0005) variants not present in in-house control individuals and known or presumed to cause isolated or syndromic CAKUT in humans or mice (Supplementary Table 2), we identified a very rare (MAF = 0.000333 according to gnomAD controls) missense variant in the DACT1 gene, NM_016651.5(DACT1):c.1100C > A p.(Thr367Lys). The gene discussed is DACT1; the disease is congenital anomaly of kidney and urinary tract.