Moreover, in three families we observed co-segregation of DACT1 variants with CAKUT or extrarenal phenotypes, i.e., megacystis in T004-II.03 and T004-II.04, kidney anomalies in H402-I.02 and H402-II.01, and skeletal anomalies in N032-I.02 and N032-II.01 (not all family members were available for genetic testing or phenotypic evaluation; Fig. 1, Supplementary Table 4). Here, DACT1 is linked to Megacystis.