Both in LN229 and U‐87 glioma cell lines, 1A‐116 specifically inhibited Rac Family Small GTPase 1 (RAC1) activation to guanine exchange factors such as T‐lymphoma invasion and metastasis‐inducing protein 1 (Tiam1), by interacting with the Trp56 residue.110, 111. This evidence concerns the gene RAC1 and central nervous system cancer.