In the present ALI model, we found that ALI compared with LF during pressure support ventilation: (1) the DAD score decreased more, mainly due to edema in axial and alveolar parenchyma; (2) birefringence for occludin and claudin‐4 in lung tissue was increased; and (3) neutrophil gelatinase‐associated lipocalin expression in kidney tissue increased more. The gene discussed is OCLN; the disease is acute respiratory distress syndrome.