Finally, U251 and U373 cells were applied in GBM transplantation in nude mice, which demonstrated that overexpression of MBNL1 and circNTRK2 combined with PAX5 knockdown effectively inhibits the formation of GBM cell xenografts, as well as significantly prolongs the survival of orthotopic nude mice. This evidence concerns the gene MBNL1 and glioblastoma.