Additionally, evaluation of Dox distribution in frozen tumor tissue sections using fluorescence microscopy indicated increased red fluorescence in the sh-METTL3, sh-IGF2BP3, CPEB2-Mut, sh-SRSF5, and sh-P51-ETS1 group, indicating that knockdown of these molecules increased the amount of Dox crossing the BTB into the glioma (Fig. 8d). The gene discussed is SRSF5; the disease is glioma.