To further evaluate the regulatory effects of these factors on BTB permeability, we used GBM orthotopic xenograft mice to demonstrate that mutation of m6A sites in CPEB2 mRNA and METTL3, SRSF5, and P51-ETS1 knockdown in GECs combined with Dox administration significantly increased BTB permeability, significantly reduced the size of GBM-transplanted tumors, prolonged survival, and increased the amount of Dox crossing the BTB into tumors. This evidence concerns the gene CPEB2 and glioblastoma.