Additionally, evaluation of Dox distribution in frozen tumor tissue sections using fluorescence microscopy indicated increased red fluorescence in the sh-METTL3, sh-IGF2BP3, CPEB2-Mut, sh-SRSF5, and sh-P51-ETS1 group, indicating that knockdown of these molecules increased the amount of Dox crossing the BTB into the glioma (Fig. 8d). This evidence concerns the gene METTL3 and central nervous system cancer.