Notably, compared to vehicle-treated controls, mice treated with Thiamet-G showed decreased blood ammonia during acute liver failure (Fig. 5d) despite similar levels of serum ALT observed between the two groups (Fig. 5e) and increased O-GlcNAcylation of CPS1 (Fig. 5f), thus suggesting that reduction of blood ammonia by Thiamet-G was not dependent on decreased liver damage. This evidence concerns the gene GPT and acute liver failure.