In order to study the long-term effect of dCas9-Tet1CD-induced demethylation on the growth of ESCC cells in vivo, we established a subcutaneous xenograft model by inoculating Kyse-30 cells with targeted demethylation or ZNF154 overexpression in BALB/c mice. Here, ZNF154 is linked to esophageal squamous cell carcinoma.