Furthermore, ESCC cell lines treated with 5-aza-2’ deoxycytidine (5-aza-dC), a potent inhibitor of DNA methyltransferase, showed a marked reduction in the methylation level of ZNF154 promoter with a concomitant upregulation of ZNF154 mRNA (Fig. 2B, C). The gene discussed is ZNF154; the disease is esophageal squamous cell carcinoma.