The comprehensive bioinformatics analysis showed that low expression of MEF2C-AS1 in CRC might lead to downregulation of 10 target mRNAs including KCNB1, CFL2, FAM129A, CLIP4, CYBRD1, SLC16A9, TMEM100, BVES, TRPM6, and ZEB1 through upregulating 3 miRNAs, hsa-miR-17-5p, hsa-miR-24-3p, and hsa-miR-429 (Additional file 1: Table S1). The gene discussed is CYBRD1; the disease is colorectal carcinoma.