MEF2C and colorectal carcinoma: After demethylation treatment with different concentrations of 5-aza-2'-deoxycytidine (5-Aza-dC) to the CRC cell line with moderate MEF2C-AS1 downregulation, RKO cell line, its expression was significantly rescued in the 15 and 20 μM groups compared with the 0 μM group, and the effect showed a dose-dependent manner (Fig. 7b, all p < 0.05).